Laboratory Engineer Multiplex Immunohistochemistry

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About the position Kromnigon AB is a biotechnology company developing reagents and technologies for multicolour microscopy and tissue-based analysis. We are seeking a Laboratory Engineer for a three-month full-time project focused on the development, optimisation and evaluation of multiplex immunohistochemistry assays on tissue samples. The position is highly laboratory-based and requires substantial hands-on experience with histological tissue preparation and immunohistochemical staining. The successful candidate will work independently with assay execution, troubleshooting and evaluation in close collaboration with the Kromnigon research team. Occupation classification: SSYK 3215 – Laboratory Engineer Main responsibilities The work will primarily include: Development, optimisation and evaluation of multiplex immunohistochemistry (IHC) assays Performance of multiplex staining protocols on tissue sections Preparation and handling of formalin-fixed paraffin-embedded (FFPE) tissue Tissue sectioning and preparation of slides for histological analysis Deparaffinisation, antigen retrieval, blocking and immunostaining Optimisation of antibodies, staining conditions and assay parameters Evaluation of staining quality, specificity and reproducibility Microscopy-based assessment and documentation of experimental results Testing and evaluation of reagents and laboratory protocols Documentation of experimental procedures and results Required qualifications We are looking for a candidate with: A relevant university degree in biomedicine, molecular biology, biotechnology, biomedical laboratory science, histology, pathology or a related life-science field Documented hands-on laboratory experience with immunohistochemistry Practical experience with multiplex immunohistochemistry and/or multiplex immunofluorescence Experience working with FFPE tissue samples Practical experience with histological techniques, including tissue sectioning, deparaffinisation and preparation of tissue sections for staining Experience in optimisation and troubleshooting of immunostaining protocols Ability to independently plan, perform, document and evaluate laboratory experiments Good written and spoken English Meritorious qualifications It is considered an advantage if the candidate has: Experience from cancer research, tumour biology or tumour pathology Experience analysing human tumour tissue Experience with multicolour fluorescence microscopy Experience with image-based evaluation of multiplex-stained tissue Previous experience developing or validating histological or immunohistochemical assays Experience working in a research or biotechnology environment Employment The position is a full-time fixed-term employment for three months, based at Kromnigon AB at AstraZeneca BioVentureHub in Mölndal, Sweden. Planned starting date: as soon as possible / by agreement.

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